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Overview
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Product name
Anti-RBM15B antibody [EPR25177-158]
See all RBM15B primary antibodies -
Description
Rabbit monoclonal [EPR25177-158] to RBM15B
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Host species
Rabbit
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Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa transfected with scrambled siRNA control, whole cell lysate; His-tagged human RBM15B recombinant protein; HeLa, Jurkat, and 293T whole cell lysates.ICC/IF: HeLa cells.Flow Cyt (Intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
![Recombinant Anti-RBM15B antibody [EPR25177-158] (ab300465) (2)](data:image/gif;base64,R0lGODlhAQABAAAAACH5BAEKAAEALAAAAAABAAEAAAICTAEAOw== "Recombinant Anti-RBM15B antibody [EPR25177-158] (ab300465) (2)")
Concentration information loading... -
Purity
Protein A purified
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Clonality
Monoclonal
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Clone number
EPR25177-158
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Isotype
IgG
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Research areas
- Signal Transduction
- Protein Trafficking
- Nuclear Import / Export
- Epigenetics and Nuclear Signaling
- DNA / RNA
- RNA Processing
- Splicing
- Epigenetics and Nuclear Signaling
- Chromatin Binding Proteins
- DNA / RNA binding
![Recombinant Anti-RBM15B antibody [EPR25177-158] (ab300465) (2)](https://i0.wp.com/www.abcam.com/ps/images/please_wait_small.gif "Recombinant Anti-RBM15B antibody [EPR25177-158] (ab300465) (2)")
Associated products
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Alternative Versions
- Anti-RBM15B antibody [EPR25177-158] (BSA and Azide free) (ab300466)
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Compatible Secondaries
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Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
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Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. | |
| WB | 1/1000. Predicted molecular weight: 97 kDa. | |
| Flow Cyt (Intra) | 1/500. |
| Notes |
|---|
| ICC/IF |
| WB |
| Flow Cyt (Intra) |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
May function in the regulation of alternative or illicit splicing.
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Tissue specificity
Ubiquitously expressed.
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Sequence similarities
Belongs to the RRM Spen family.
Contains 3 RRM (RNA recognition motif) domains.
Contains 1 SPOC domain. -
Cellular localization
Nucleus > nucleoplasm. Colocalizes with BMLF1 in the nucleus. Localized in the nucleoplasm with a granular staining pattern and excluded from the nucleoli.
- Information by UniProt
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Database links
- Entrez Gene: 29890 Human
- Omim: 612602 Human
- SwissProt: Q8NDT2 Human
- Unigene: 118738 Human
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Alternative names
- 1810017N16Rik antibody
- Chromosome 3p21.1 gene sequence antibody
- HUMAGCGB antibody
see all
Images
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Western blot - Anti-RBM15B antibody [EPR25177-158] (AB300465)
All lanes : Anti-RBM15B antibody [EPR25177-158] (ab300465) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting RBM15B, whole cell lysate 'a'
Lane 3 : HeLa transfected with siRNA specifically targeting RBM15B, whole cell lysate 'b'Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionPredicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Exposure time: 114 seconds
Blocking / Diluting buffer: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:27602518).
The bands below 100 kDa may be caused by degradation.
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Western blot - Anti-RBM15B antibody [EPR25177-158] (AB300465)
All lanes : Anti-RBM15B antibody [EPR25177-158] (ab300465) at 1/1000 dilution
Lane 1 : His-tagged human RBM15B recombinant protein, 10 ng
Lane 2 : His-tagged human RBM15 recombinant protein, 10 ngSecondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutionPredicted band size: 97 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking / Diluting buffer: 5% NFDM/TBST.
This antibody does not cross-react with human RBM15.
Both recombinant proteins were expressed from E.coli expression systems.
Exposure time 7.75 seconds.
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Western blot - Anti-RBM15B antibody [EPR25177-158] (AB300465)
All lanes : Anti-RBM15B antibody [EPR25177-158] (ab300465) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysateLysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutionPredicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Exposure time: 180 seconds
Blocking / Diluting buffer: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands below 100 kDa may be caused by degradation.
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Western blot - Anti-RBM15B antibody [EPR25177-158] (AB300465)
Anti-RBM15B antibody [EPR25177-158] (ab300465) at 1/1000 dilution + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutionPredicted band size: 97 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Exposure time: 92 seconds
Blocking / Diluting buffer: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands below 100 kDa may be caused by degradation.
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Immunocytochemistry/ Immunofluorescence - Anti-RBM15B antibody [EPR25177-158] (ab300465)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cells) labelling RBM15B with ab300465 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line. ab195889. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used as a counterstain at 1/200 dilution (red). The nuclear counterstain is DAPI (blue).
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Flow Cytometry (Intracellular) - Anti-RBM15B antibody [EPR25177-158] (AB300465)
Flow Cytometry (Intracellular) analysis of HeLa (human cervix adenocarcinoma epithelial cells) labeling RBM15B (red) with ab300465 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730) Isotype control. Blue - (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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Anti-RBM15B antibody [EPR25177-158] (AB300465)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Click here to view the general protocols
Datasheets and documents
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SDS download
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Datasheet download
Download
Certificate of Compliance
References (0)
Publishing research using ab300465? Please let us know so that we can cite the reference in this datasheet.
ab300465 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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